Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment.
Identifieur interne : 002268 ( Main/Exploration ); précédent : 002267; suivant : 002269Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment.
Auteurs : Qining Sun [États-Unis] ; Marcus Foston [États-Unis] ; Xianzhi Meng [États-Unis] ; Daisuke Sawada [États-Unis] ; Sai Venkatesh Pingali [États-Unis] ; Hugh M. O'Neill [États-Unis] ; Hongjia Li [États-Unis] ; Charles E. Wyman [États-Unis] ; Paul Langan [États-Unis] ; Art J. Ragauskas [États-Unis] ; Rajeev Kumar [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2014.
Abstract
BACKGROUND
Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol production is thermochemical pretreatment to reduce plant cell wall recalcitrance for downstream processes. Previous studies of dilute acid pretreatment (DAP) have shown significant changes in cellulose ultrastructure that occur during pretreatment, but there is still a substantial knowledge gap with respect to the influence of lignin on these cellulose ultrastructural changes. This study was designed to assess how the presence of lignin influences DAP-induced changes in cellulose ultrastructure, which might ultimately have large implications with respect to enzymatic deconstruction efforts.
RESULTS
Native, untreated hybrid poplar (Populus trichocarpa x Populus deltoids) samples and a partially delignified poplar sample (facilitated by acidic sodium chlorite pulping) were separately pretreated with dilute sulfuric acid (0.10 M) at 160°C for 15 minutes and 35 minutes, respectively . Following extensive characterization, the partially delignified biomass displayed more significant changes in cellulose ultrastructure following DAP than the native untreated biomass. With respect to the native untreated poplar, delignified poplar after DAP (in which approximately 40% lignin removal occurred) experienced: increased cellulose accessibility indicated by increased Simons' stain (orange dye) adsorption from 21.8 to 72.5 mg/g, decreased cellulose weight-average degree of polymerization (DPw) from 3087 to 294 units, and increased cellulose crystallite size from 2.9 to 4.2 nm. These changes following DAP ultimately increased enzymatic sugar yield from 10 to 80%.
CONCLUSIONS
Overall, the results indicate a strong influence of lignin content on cellulose ultrastructural changes occurring during DAP. With the reduction of lignin content during DAP, the enlargement of cellulose microfibril dimensions and crystallite size becomes more apparent. Further, this enlargement of cellulose microfibril dimensions is attributed to specific processes, including the co-crystallization of crystalline cellulose driven by irreversible inter-chain hydrogen bonding (similar to hornification) and/or cellulose annealing that converts amorphous cellulose to paracrystalline and crystalline cellulose. Essentially, lignin acts as a barrier to prevent cellulose crystallinity increase and cellulose fibril coalescence during DAP.
DOI: 10.1186/s13068-014-0150-6
PubMed: 25342973
PubMed Central: PMC4205766
Affiliations:
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Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, 900 University Avenue, Riverside, CA 92521 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, 900 University Avenue, Riverside, CA 92521 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Langan, Paul" sort="Langan, Paul" uniqKey="Langan P" first="Paul" last="Langan">Paul Langan</name><affiliation wicri:level="2"><nlm:affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Ragauskas, Art J" sort="Ragauskas, Art J" uniqKey="Ragauskas A" first="Art J" last="Ragauskas">Art J. Ragauskas</name><affiliation wicri:level="2"><nlm:affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA ; Department of Chemical and Biomolecular Engineering, Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee, Knoxville, TN 37996-2200 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA ; Department of Chemical and Biomolecular Engineering, Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee, Knoxville</wicri:cityArea></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25342973</idno><idno type="pmid">25342973</idno><idno type="doi">10.1186/s13068-014-0150-6</idno><idno type="pmc">PMC4205766</idno><idno type="wicri:Area/Main/Corpus">001F53</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001F53</idno><idno type="wicri:Area/Main/Curation">001F53</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001F53</idno><idno type="wicri:Area/Main/Exploration">001F53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment.</title><author><name sortKey="Sun, Qining" sort="Sun, Qining" uniqKey="Sun Q" first="Qining" last="Sun">Qining Sun</name><affiliation wicri:level="2"><nlm:affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Foston, Marcus" sort="Foston, Marcus" uniqKey="Foston M" first="Marcus" last="Foston">Marcus Foston</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy, Environmental and Chemical Engineering, Washington University, 1 Brookings Drive, Saint Louis, MO 63130 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Department of Energy, Environmental and Chemical Engineering, Washington University, 1 Brookings Drive, Saint Louis</wicri:cityArea></affiliation></author><author><name sortKey="Meng, Xianzhi" sort="Meng, Xianzhi" uniqKey="Meng X" first="Xianzhi" last="Meng">Xianzhi Meng</name><affiliation wicri:level="2"><nlm:affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Sawada, Daisuke" sort="Sawada, Daisuke" uniqKey="Sawada D" first="Daisuke" last="Sawada">Daisuke Sawada</name><affiliation wicri:level="2"><nlm:affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Pingali, Sai Venkatesh" sort="Pingali, Sai Venkatesh" uniqKey="Pingali S" first="Sai Venkatesh" last="Pingali">Sai Venkatesh Pingali</name><affiliation wicri:level="2"><nlm:affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="O Neill, Hugh M" sort="O Neill, Hugh M" uniqKey="O Neill H" first="Hugh M" last="O'Neill">Hugh M. O'Neill</name><affiliation wicri:level="2"><nlm:affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Li, Hongjia" sort="Li, Hongjia" uniqKey="Li H" first="Hongjia" last="Li">Hongjia Li</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, 900 University Avenue, Riverside, CA 92521 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, 900 University Avenue, Riverside, CA 92521 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Langan, Paul" sort="Langan, Paul" uniqKey="Langan P" first="Paul" last="Langan">Paul Langan</name><affiliation wicri:level="2"><nlm:affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Ragauskas, Art J" sort="Ragauskas, Art J" uniqKey="Ragauskas A" first="Art J" last="Ragauskas">Art J. Ragauskas</name><affiliation wicri:level="2"><nlm:affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA ; Department of Chemical and Biomolecular Engineering, Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee, Knoxville, TN 37996-2200 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA ; Department of Chemical and Biomolecular Engineering, Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee, Knoxville</wicri:cityArea></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol production is thermochemical pretreatment to reduce plant cell wall recalcitrance for downstream processes. Previous studies of dilute acid pretreatment (DAP) have shown significant changes in cellulose ultrastructure that occur during pretreatment, but there is still a substantial knowledge gap with respect to the influence of lignin on these cellulose ultrastructural changes. This study was designed to assess how the presence of lignin influences DAP-induced changes in cellulose ultrastructure, which might ultimately have large implications with respect to enzymatic deconstruction efforts.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Native, untreated hybrid poplar (Populus trichocarpa x Populus deltoids) samples and a partially delignified poplar sample (facilitated by acidic sodium chlorite pulping) were separately pretreated with dilute sulfuric acid (0.10 M) at 160°C for 15 minutes and 35 minutes, respectively . Following extensive characterization, the partially delignified biomass displayed more significant changes in cellulose ultrastructure following DAP than the native untreated biomass. With respect to the native untreated poplar, delignified poplar after DAP (in which approximately 40% lignin removal occurred) experienced: increased cellulose accessibility indicated by increased Simons' stain (orange dye) adsorption from 21.8 to 72.5 mg/g, decreased cellulose weight-average degree of polymerization (DPw) from 3087 to 294 units, and increased cellulose crystallite size from 2.9 to 4.2 nm. These changes following DAP ultimately increased enzymatic sugar yield from 10 to 80%.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Overall, the results indicate a strong influence of lignin content on cellulose ultrastructural changes occurring during DAP. With the reduction of lignin content during DAP, the enlargement of cellulose microfibril dimensions and crystallite size becomes more apparent. Further, this enlargement of cellulose microfibril dimensions is attributed to specific processes, including the co-crystallization of crystalline cellulose driven by irreversible inter-chain hydrogen bonding (similar to hornification) and/or cellulose annealing that converts amorphous cellulose to paracrystalline and crystalline cellulose. Essentially, lignin acts as a barrier to prevent cellulose crystallinity increase and cellulose fibril coalescence during DAP.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25342973</PMID><DateCompleted><Year>2014</Year><Month>10</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment.</ArticleTitle><Pagination><MedlinePgn>150</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-014-0150-6</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol production is thermochemical pretreatment to reduce plant cell wall recalcitrance for downstream processes. Previous studies of dilute acid pretreatment (DAP) have shown significant changes in cellulose ultrastructure that occur during pretreatment, but there is still a substantial knowledge gap with respect to the influence of lignin on these cellulose ultrastructural changes. This study was designed to assess how the presence of lignin influences DAP-induced changes in cellulose ultrastructure, which might ultimately have large implications with respect to enzymatic deconstruction efforts.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Native, untreated hybrid poplar (Populus trichocarpa x Populus deltoids) samples and a partially delignified poplar sample (facilitated by acidic sodium chlorite pulping) were separately pretreated with dilute sulfuric acid (0.10 M) at 160°C for 15 minutes and 35 minutes, respectively . Following extensive characterization, the partially delignified biomass displayed more significant changes in cellulose ultrastructure following DAP than the native untreated biomass. With respect to the native untreated poplar, delignified poplar after DAP (in which approximately 40% lignin removal occurred) experienced: increased cellulose accessibility indicated by increased Simons' stain (orange dye) adsorption from 21.8 to 72.5 mg/g, decreased cellulose weight-average degree of polymerization (DPw) from 3087 to 294 units, and increased cellulose crystallite size from 2.9 to 4.2 nm. These changes following DAP ultimately increased enzymatic sugar yield from 10 to 80%.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Overall, the results indicate a strong influence of lignin content on cellulose ultrastructural changes occurring during DAP. With the reduction of lignin content during DAP, the enlargement of cellulose microfibril dimensions and crystallite size becomes more apparent. Further, this enlargement of cellulose microfibril dimensions is attributed to specific processes, including the co-crystallization of crystalline cellulose driven by irreversible inter-chain hydrogen bonding (similar to hornification) and/or cellulose annealing that converts amorphous cellulose to paracrystalline and crystalline cellulose. Essentially, lignin acts as a barrier to prevent cellulose crystallinity increase and cellulose fibril coalescence during DAP.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Qining</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Foston</LastName><ForeName>Marcus</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Energy, Environmental and Chemical Engineering, Washington University, 1 Brookings Drive, Saint Louis, MO 63130 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Xianzhi</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sawada</LastName><ForeName>Daisuke</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pingali</LastName><ForeName>Sai Venkatesh</ForeName><Initials>SV</Initials><AffiliationInfo><Affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>O'Neill</LastName><ForeName>Hugh M</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hongjia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, 900 University Avenue, Riverside, CA 92521 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Langan</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Center for Structural Molecular Biology and the Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>Art J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>School of Chemistry and Biochemistry, Renewable Bioproducts Institute, Georgia Institute of Technology, 500 10th Street, N.W. Atlanta, GA 30332-0620 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA ; Department of Chemical and Biomolecular Engineering, Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee, Knoxville, TN 37996-2200 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>10</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biomass recalcitrance</Keyword><Keyword MajorTopicYN="N">Cellulose ultrastructure</Keyword><Keyword MajorTopicYN="N">Delignification</Keyword><Keyword MajorTopicYN="N">Dilute acid pretreatment</Keyword><Keyword MajorTopicYN="N">Enzymatic sugar release</Keyword><Keyword MajorTopicYN="N">Lignin content</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>05</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>09</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25342973</ArticleId><ArticleId IdType="doi">10.1186/s13068-014-0150-6</ArticleId><ArticleId IdType="pii">150</ArticleId><ArticleId IdType="pmc">PMC4205766</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Carbohydr Res. 2006 Apr 10;341(5):591-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16442511</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Prog. 2008 Sep-Oct;24(5):1178-85</Citation><ArticleIdList><ArticleId IdType="pubmed">19194930</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2012 Mar;107:235-42</Citation><ArticleIdList><ArticleId IdType="pubmed">22243924</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2007 Sep 1;98(1):112-22</Citation><ArticleIdList><ArticleId IdType="pubmed">17335064</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1983 Jan;25(1):33-51</Citation><ArticleIdList><ArticleId IdType="pubmed">18548537</ArticleId></ArticleIdList></Reference><Reference><Citation>Solid State Nucl Magn Reson. 1999 Oct;15(1):31-40</Citation><ArticleIdList><ArticleId IdType="pubmed">10903082</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomacromolecules. 2011 Jan 10;12 (1):194-202</Citation><ArticleIdList><ArticleId IdType="pubmed">21133402</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1984 Oct;26(10):1219-22</Citation><ArticleIdList><ArticleId IdType="pubmed">18551639</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2007 Feb 9;315(5813):804-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17289988</ArticleId></ArticleIdList></Reference><Reference><Citation>ChemSusChem. 2009;2(12):1096-107</Citation><ArticleIdList><ArticleId IdType="pubmed">19950346</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2008 Sep 1;101(1):39-48</Citation><ArticleIdList><ArticleId IdType="pubmed">18421796</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2013 Sep;144:467-76</Citation><ArticleIdList><ArticleId IdType="pubmed">23899571</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Jul 18;8(7):e68266</Citation><ArticleIdList><ArticleId IdType="pubmed">23874568</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2010 Oct;101(19):7410-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20471250</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Res. 2005 Jan 17;340(1):97-106</Citation><ArticleIdList><ArticleId IdType="pubmed">15620672</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2007 Sep;98(13):2503-10</Citation><ArticleIdList><ArticleId IdType="pubmed">17113771</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2011 Dec 20;4(1):60</Citation><ArticleIdList><ArticleId IdType="pubmed">22185437</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomacromolecules. 2004 Jul-Aug;5(4):1333-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15244448</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS J. 2010 Mar;277(6):1571-82</Citation><ArticleIdList><ArticleId IdType="pubmed">20148968</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2013 Jan 28;6(1):16</Citation><ArticleIdList><ArticleId IdType="pubmed">23356676</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2013 Jan 28;6(1):15</Citation><ArticleIdList><ArticleId IdType="pubmed">23356640</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Res. 2009 Sep 28;344(14 ):1879-900</Citation><ArticleIdList><ArticleId IdType="pubmed">19616198</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2009 Sep;100(17):3948-62</Citation><ArticleIdList><ArticleId IdType="pubmed">19362819</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Prog. 2001 Nov-Dec;17(6):1049-54</Citation><ArticleIdList><ArticleId IdType="pubmed">11735439</ArticleId></ArticleIdList></Reference><Reference><Citation>Angew Chem Int Ed Engl. 2005 May 30;44(22):3358-93</Citation><ArticleIdList><ArticleId IdType="pubmed">15861454</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2011 Feb 10;4:3</Citation><ArticleIdList><ArticleId IdType="pubmed">21310050</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Prog. 1999 Oct 1;15(5):804-816</Citation><ArticleIdList><ArticleId IdType="pubmed">10514250</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Géorgie (États-Unis)</li><li>Missouri (État)</li><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Sun, Qining" sort="Sun, Qining" uniqKey="Sun Q" first="Qining" last="Sun">Qining Sun</name></region><name sortKey="Foston, Marcus" sort="Foston, Marcus" uniqKey="Foston M" first="Marcus" last="Foston">Marcus Foston</name><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><name sortKey="Langan, Paul" sort="Langan, Paul" uniqKey="Langan P" first="Paul" last="Langan">Paul Langan</name><name sortKey="Li, Hongjia" 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O'Neill</name><name sortKey="Pingali, Sai Venkatesh" sort="Pingali, Sai Venkatesh" uniqKey="Pingali S" first="Sai Venkatesh" last="Pingali">Sai Venkatesh Pingali</name><name sortKey="Ragauskas, Art J" sort="Ragauskas, Art J" uniqKey="Ragauskas A" first="Art J" last="Ragauskas">Art J. Ragauskas</name><name sortKey="Sawada, Daisuke" sort="Sawada, Daisuke" uniqKey="Sawada D" first="Daisuke" last="Sawada">Daisuke Sawada</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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